What is a good B-factor?

What is a good B-factor?

B-factor is, in general, correlated with the resolution of your datasets. In the 3 to 5 angstrom resolution range, isotropic atomic B-factors can be 100, 200 or greater. At higher resolution, B-factors from 20-100 are reasonable.

What does B-factor tell you?

Roughly speaking, B-factors indicate the precision of the atom positions. Atom positions can be uncertain because of disorder in the crystal from which the structure was determined. High B-factors mean greater uncertainty about the actual atom position.

What is average B-factor?

B_max tend to increase if resolution decreases. In the highest resolution range (0.0–1.5 Å) and when all the atom types are considered together, the average B-factor at pcVol = 100% is only 25 Å2, while it is 80 Å2, in the lowest resolution range (3.3–4.0 Å).

How is B-factor calculated?

For example: The temperature of the md simulation was set at 298K. The B-factor at 298 K was calculated from RMSF by the formula: B = 8*Pi*Pi*(RMSF)**2/3 . But the temperature in pdb file is 100 K.

What is anisotropic B-factor?

An anisotropic scale factor is a multiplicative factor like the overall B-factor. Like the overall B-factor, its value varies with resolution. But, unlike the overall B-factor, its value also varies with direction.

What is Wilson B-factor?

The Wilson B factor indicates the degree of order in the crystal. It is an approximation of the average atomic B factors which may be later obtained in refinement.

What is Debye-Waller effect?

The Debye–Waller factor (DWF), named after Peter Debye and Ivar Waller, is used in condensed matter physics to describe the attenuation of x-ray scattering or coherent neutron scattering caused by thermal motion. It has also been called the B factor or the temperature factor.

What is crystallographic B-factor?

The crystallographic atomic displacement parameters, more commonly known as B-factors, are reported for all protein X-ray crystal structures and they are directly related to the atomic fluctuations (due to motion or static disorder) in the crystal.

What is Wilson plot?

The Wilson plot is a technique to estimate the film coefficients in several types of heat transfer processes and to obtain general heat transfer correlations. This method is an outstanding tool in practical applications and in laboratory research activities that involve analysis of heat exchangers.

How is Debye-Waller factor calculated?

The Debye-Waller factor is directly related to the atomic displacement parameter through a geometric scaling term; B = 8π2U.

What is crystallographic B factor?

What is a good B-factor? B-factor is, in general, correlated with the resolution of your datasets. In the 3 to 5 angstrom resolution range, isotropic atomic B-factors can be 100, 200 or greater. At higher resolution, B-factors from 20-100 are reasonable. What does B-factor tell you? Roughly speaking, B-factors indicate the precision of the atom…