What is XL1-Blue Supercompetent cells?
What is XL1-Blue Supercompetent cells?
XL1-Blue cells are endonuclease (endA) deficient, which greatly improves the quality of. miniprep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned. DNA by the EcoK endonuclease system.
What was the transformation efficiency of the E coli XL1-Blue cells used in the lab?
5 x 109
Transformation efficiency is 5 x 109 transformants/µg of pUC18 DNA. Cells are resistant to tetracycline and chloramphenicol.
What are DH5 alpha competent cells?
DH5-Alpha Cells are E. coli cells engineered by American biologist Douglas Hanahan to maximize transformation efficiency. They are defined by three mutations: recA1, endA1 which help plasmid insertion and lacZΔM15 which enables blue white screening.
How do you calculate transformation efficiency of competent cells?
Transformation efficiency is defined as the number of colony forming units (cfu) produced by 1µg of Competent Cells Control DNA (supercoiled plasmid DNA) and is measured by performing a control transformation reaction using a known quantity of DNA, typically 0.1ng, then calculating the number of cfu formed per …
Why is E coli used for transformation?
coli is a preferred host for gene cloning due to the high efficiency of introduction of DNA molecules into cells. E. coli is a preferred host for protein production due to its rapid growth and the ability to express proteins at very high levels.
How long do competent cells last?
How Long can Competent Cells be Stored. When stored and handled properly, GoldBio competent cells should be stable at -80°C for at least 1 year.
How do you handle competent cells?
General Handling of Competent Cells Competent cells must be treated gently. Mix cells by swirling or gently tapping the reaction tube. Do not mix by pipetting or vortexing. Once thawed, the cells should be used.
What is XL1-Blue Supercompetent cells? XL1-Blue cells are endonuclease (endA) deficient, which greatly improves the quality of. miniprep DNA, and are recombination (recA) deficient, improving insert stability. The hsdR mutation prevents the cleavage of cloned. DNA by the EcoK endonuclease system. What was the transformation efficiency of the E coli XL1-Blue cells used in the…