What is the optimum temperature for acid phosphatase?

30°C.
The optimal temperature of acid phosphatase was 30°C. A higher value was 37°C which was reported for the enzyme from cotton seedlings (Bhargava and Sachar, 1987).

What does acid phosphatase do in wheat germ?

Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes.

What class of enzyme does wheat germ acid phosphatase belong to?

Acid phosphatases (APs) are a family of enzymes that belong to the hydrolase class.

What is the pH of wheat germ?

The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.

How does temperature affect acid phosphatase?

The intracellular acid phosphatase activity is maximal at a growth temperature of 20°C. The level of the secreted phosphatase activity decreases as growth temperature increases from 15 to 36°C. It is the growth temperature itself and not the growth rate that regulates these activities.

Where is wheat germ acid phosphatase found?

Commercial wheat germ acid phosphatase (WGAP) is extracted from the germ/embryo of wheat plants and is commonly used in vitro in many biochemistry laboratories.

Is phosphatase a hydrolase?

In biochemistry, a phosphatase is an enzyme that uses water to cleave a phosphoric acid monoester into a phosphate ion and an alcohol. Because a phosphatase enzyme catalyzes the hydrolysis of its substrate, it is a subcategory of hydrolases.

What is the clinical significance of acid phosphatase?

Traditionally, the serum prostatic acid phosphatase has been thought to originate in the prostatic cancer cell and has been used to stage the disease. Until recently, elevated serum values have been accepted as an indication of extraprostatic disease, and were thought to rule out lesions confined to the prostate.

How does pH affect enzyme activity?

Enzymes are also sensitive to pH . Changing the pH of its surroundings will also change the shape of the active site of an enzyme. This contributes to the folding of the enzyme molecule, its shape, and the shape of the active site. Changing the pH will affect the charges on the amino acid molecules.

How to extract wheat germ acid phosphatase?

Exuaction Procedures The procedures detailed below for the extraction of wheat germ acid phosphatase are representative of a typical protein isolation scheme. The initial water ex traction of the enzyme (along with a number of other water-soluble proteins, cartJohydrates, etc.l is accom

How to clean acid phosphatase in a centrifuge?

Day 1: Acid phosphatase purification, part 1 1-1) Mix together 40 ml of cold water and 10 grams of raw wheat germ. Stir occasionally for 30 minutes on ice. 1-2) Filter the suspension into a centrifuge tube through two layers of cheesecloth placed in a funnel.

What kind of assay is acid phosphatase?

The assay reaction uses a synthetic substrate: p-nitrophenylphosphate (PNPP). It is hydrolyzed by Acid phosphatase, yielding p-nitrophenol (PNP). PNP is colorless at pH 5.6, but at pH 7.6 it is yellow. The amount of PNP formed can then be easily measured using a spectrophotometer.

What is the optimum temperature for acid phosphatase? 30°C. The optimal temperature of acid phosphatase was 30°C. A higher value was 37°C which was reported for the enzyme from cotton seedlings (Bhargava and Sachar, 1987). What does acid phosphatase do in wheat germ? Acid phosphatase originates from the aleurone and scutellar tissues during germination. It…