What is 4T1 tumor?
What is 4T1 tumor?
The 4T1 mammary carcinoma is a transplantable tumor cell line that is highly tumorigenic and invasive and, unlike most tumor models, can spontaneously metastasize from the primary tumor in the mammary gland to multiple distant sites including lymph nodes, blood, liver, lung, brain, and bone The 4T1 tumor has several …
What is 4T1 cell line?
4T1 is a breast cancer cell line derived from the mammary gland tissue of a mouse BALB/c strain. 4T1 cells are epithelial and are resistant to 6-thioguanine. In preclinical research, 4T1 cells have been used to study breast cancer metastasis as they can metastasize to the lung, liver, lymph nodes, brain and bone.
Is 4T1 triple negative?
4T1 Triple Negative Mouse Breast Cancer Cell Line and Other Preclinical Oncology Models. 4T1 is a triple-negative mouse breast cancer cell line.
What is MC38?
The MC38 murine colon carcinoma model can be employed as a robust preclinical immuno-oncology model. Our data supports the use of this tool in investigating novel treatment combinations with radiation, checkpoint inhibitors, costimulatory molecules, or other novel approaches.
What is syngeneic mouse model?
What is a syngeneic mouse model? Syngeneic mouse models, also known as Allograft mouse tumor systems, consist of tumor tissues derived from the same genetic background as a given mouse strain. As the syngeneic mice retain intact immune systems, they are particularly relevant for studies of immunotherapies.
What is MC38 mouse model?
Model Information: MC38 is derived from a colon tumor in a C57BL/6 mouse following long-term exposure to 1,2-dimethylhydrazine dihydrochloride (DMH)(1). It is one of the most popularly used model for the assessment of anti-cancer immunotherapies and is fit for the evaluation of efficacy and PD effects.
What is a mouse xenograft?
Patient‑derived xenograft (PDX) mouse models involve the direct transfer of fresh human tumor samples into immunodeficient mice following surgical resection or other medical operations. Gene expression in tumors may be maintained by serial passages of tumors from mouse to mouse.
What is an orthotopic mouse model?
Orthotopic models involve the seeding of tumor cell lines into the corresponding tissue in animal models. This strategy allows us to assess tumor development in a relevant environment and evaluate efficacy in a preclinical tumor model that mimics the disease process in humans.
What is HT cell?
HT is a human B cell lymphoma cell line originated in 1983 by Walter J. HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface. Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
What are RKO cells?
RKO is a poorly differentiated colon carcinoma cell line developed by Michael Brattain. The RKO cell line is the parental cell line (isogenic) of RKO-E6 (ATCC CRL-2578) and RKO-A545-1 (ATCC CRL-2579).
What is the meaning of syngeneic?
(SIN-jeh-NAY-ik) Having to do with individuals or tissues that have identical genes. For example, identical twins and cells and tissues from them are syngeneic.
How are 4T1 mouse mammary tumor cells cultured?
The 67NR, 168FARN and 4T1 mouse mammary tumor cell lines were obtained from Dr. Fred Miller at Karmanos Cancer Institute. Cells were cultured in high glucose DMEM supplemented 5% FBS, 5% NCS, NEAA and antibiotics (100 units/ml penicillin and 100 μg/ml streptomycin) at 37°C in a humidified atmosphere containing 5% CO 2.
Is there a 4T1 breast cancer cell line?
[…] The 4T1 mouse mammary tumor cell line is one of only a few breast cancer models with the capacity to metastasize efficiently to sites affected in human breast cancer. Here we describe two 4T1 cell lines modified to facilitate analysis of tumor growth and metastasis and evaluation of gene function in vivo.
What are the differences in the imagable 4T1 cell line?
Genes that differed significantly (p < 0.0001) by > 2-fold for both comparisons (4T1/67NR and 4T1/168FARN) were considered those associated with the metastatic phenotype of the 4T1 cell line.
How long does it take for 4t1-12b cells to implant?
Imaging of animals and organs at six weeks. 4T1-12B cells (10 6) were implanted into the mammary fat pad of a normal female BALB/c mouse. After six weeks the whole animal and organs were imaged as described in MATERIALS AND METHODS.
What is 4T1 tumor? The 4T1 mammary carcinoma is a transplantable tumor cell line that is highly tumorigenic and invasive and, unlike most tumor models, can spontaneously metastasize from the primary tumor in the mammary gland to multiple distant sites including lymph nodes, blood, liver, lung, brain, and bone The 4T1 tumor has several ……