What does Caco-2 stand for?
What does Caco-2 stand for?
human colon adenocarcinoma
4.5. Caco-2 cell (human colon adenocarcinoma) monolayers have been widely used as an in vitro model to evaluate the absorption enhancement across the intestinal epithelium by assessing their TEER. The cell was treated with fresh cell culture medium and medium was also added to basal compartments.
What is Caco-2 cell line?
Caco-2 is an immortalized cell line of human colorectal adenocarcinoma cells. It is primarily used as a model of the intestinal epithelial barrier. In culture, Caco-2 cells spontaneously differentiate into a heterogeneous mixture of intestinal epithelial cells.
Why are Caco-2 cells used?
Caco-2 cells are a human colon epithelial cancer cell line used as a model of human intestinal absorption of drugs and other compounds. When cultured as a monolayer, Caco-2 cells differentiate to form tight junctions between cells to serve as a model of paracellular movement of compounds across the monolayer.
Where do Caco-2 cells come from?
The Caco-2 cell line is originally derived from a colon carcinoma. However, one of its most advantageous properties is its ability to spontaneously differentiate into a monolayer of cells with many properties typical of absorptive enterocytes with brush border layer as found in the small intestine.
How do Caco-2 cells grow?
The cells should be kept at 37 °C in a humidified atmosphere containing 5 % CO2. For propagation in culture flasks, Caco-2 cells are seeded in a concentration of 105 cells/cm2. Medium should be changed every 3 days. At 80 % confluence, typically after 4–5 days, the cells are split 1:10 before further cultivation.
What is a Caco-2 permeability assay?
Cyprotex’s Caco-2 permeability assay uses an established method that measures the rate of flux of a compound across polarised Caco-2 cell monolayers and from which the data generated can be used to predict in vivo absorption of drugs. The Caco-2 cell line is derived from a human colon carcinoma.
How do you differentiate Caco-2 cells?
Standard methods used to differentiate Caco-2 cells involve seeding them on culture inserts fitted with polycarbonate filters, and allowing spontaneous differentiation to proceed for two to three weeks in culture medium containing 10% or 20% foetal bovine serum (FBS) in both the apical (AP) and basolateral (BL) …
Are Caco-2 cells adherent?
What is permeability Caco-2?
The human colon epithelial cancer cell line, Caco-2, is used as a model of human intestinal absorption of drugs. This model is suitable to test compound suitability for oral dosing, predict intestinal permeability and investigate drug efflux.
What is CT26?
CT26 is a murine colorectal carcinoma cell line which is from a BALB/c mouse. The cell is a clone of the N-nitroso-N-methylrethane-induced undifferentiated CT26 colon carcinoma cell line. These cells are adherent and have a fibroblast morphology.
What are Caco 2 cells?
The Caco-2 cell line is a continuous line of heterogeneous human epithelial colorectal adenocarcinoma cells, developed by the Sloan-Kettering Institute for Cancer Research through research conducted by Dr. Jorgen Fogh.
When did Jorgen Fogh invent Caco 2?
In culture, Caco-2 cells spontaneously differentiate into a heterogeneous mixture of intestinal epithelial cells. It was developed in 1977 by Jorgen Fogh at the Sloan-Kettering Institute for Cancer Research.
How is the Caco-2 monolayer used in medicine?
The Caco-2 monolayer is widely used across the pharmaceutical industry as an in vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs with different kits such as the CacoReady.
Which is a reference compound for Caco-2 permeability assay?
Two reference compounds, atenolol (passive paracellular transport) and propranolol (passive transcellular transport) are screened alongside the test compounds. Atenolol and propranolol have known human absorption of 50% and 90% respectively 2,3, and can be used as markers for ranking test compounds.
What does Caco-2 stand for? human colon adenocarcinoma 4.5. Caco-2 cell (human colon adenocarcinoma) monolayers have been widely used as an in vitro model to evaluate the absorption enhancement across the intestinal epithelium by assessing their TEER. The cell was treated with fresh cell culture medium and medium was also added to basal compartments. What…