How to calculate MESF?
How to calculate MESF?
Q. How do you determine the MESF value per antibody? A. This can be determined by using a microbead that has a calibrated number of binding sites for the antibody and finding its MESF value when it is saturated with the antibody, then dividing its MESF value by the number of binding sites on the microbead.
What is MESF?
MESF stands for Molecules of Equivalent Soluble Fluorochrome. Each microsphere population in a Quantum™ MESF kit is assigned an MESF value. The MESF unit corresponds to the fluorescence intensity of a given number of pure fluorochrome molecules in solution.
What is MESF in flow cytometry?
MESF is an abbreviation for Molecules of Equivalent Soluble Fluorochrome. The MESF concept indicates that a sample labeled with a fluorochrome has the same fluorescence intensity as an equivalent num- ber of molecules of the fluorochrome free in a solution under the same environmental conditions.
What are MESF beads?
Our Quantum™ MESF and Quantum™ Simply Cellular® beads are external standards that enable the standardization of fluorescence intensity units irrespective of cytometer and software. Because they are labeled with the same fluorochromes used to label cells, they provide a synchronous response to the environment.
Is flow cytometry quantitative?
An ultimate goal of quantitative flow cytometry is to measure the number of antigens or ligand-binding sites associated with a cell by measuring the number of antibodies bound per cell (ABC).
How are MESF units on the standards determined?
A. Fluorescence intensity can be quantified by using standard units known as Molecules of Equivalent Soluble Fluorochrome (MESF). Q. How are MESF units on the standards determined?
What does MESF stand for in quantum system?
MESF stands for Molecules of Equivalent Soluble Fluorochrome. Each microsphere population in a Quantum™ MESF kit is assigned an MESF value. The MESF unit corresponds to the fluorescence intensity of a given number of pure fluorochrome molecules in solution.
How is the fluorescence intensity of a MESF determined?
A. MESF units are determined by comparing the fluorescence intensity signal from the microbead standards to the signal from a solution of the same fluorochrome. Direct comparisons can be made in a spectrofluorometer between fluorochrome solutions and suspensions of microbead standards as long as the spectra match.
What does MESF stand for in flow cytometry?
Over the past twenty years, the fluorescence unit of intensity, MESF, has been introduced and utilized in the field of flow cytometry. MESF is an abbreviation for Molecules of Equivalent Soluble Fluorochrome.
How to calculate MESF? Q. How do you determine the MESF value per antibody? A. This can be determined by using a microbead that has a calibrated number of binding sites for the antibody and finding its MESF value when it is saturated with the antibody, then dividing its MESF value by the number of…