What does elongation factor Tu Do?

Elongation factor thermal unstable Tu (EF-Tu) is a G protein that catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome inside living cells. Structural and biochemical studies have described the complex interactions needed to effect canonical function.

What is the function of elongation factor Ts in elongation?

EF-Tu (elongation factor thermo unstable) is a prokaryotic elongation factor responsible for catalyzing the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome. It is a G-protein, and facilitates the selection and binding of an aa-tRNA to the A-site of the ribosome.

What are the factors involved in chain elongation?

The elongation phase of translation leads to the decoding of the mRNA and the synthesis of the corresponding polypeptide chain. In most eukaryotes, two distinct protein elongation factors (eEF-1 and eEF-2) are required for elongation.

What is a translation elongation factor?

Translation elongation factors are the workhorses of protein synthesis on the ribosome. They assist in elongating the nascent polypeptide chain by one amino acid at a time. The general biochemical outline of the translation elongation cycle is well preserved in all bio- logical kingdoms.

What is the function of elongation?

Translation elongation factors perform critical functions in protein synthesis in all domains of life, including the delivery of aminoacyl-tRNAs into the ribosome, and the translocation of peptidyl-tRNA from the ribosomal A-site to the ribosomal P-site.

What is EF-Tu made of?

EF-Tu is suggested to consist of three domains: 1 (amino acids 1–200), 2 (amino acids 208–295), and 3 (amino acids 298–394) (36), all of them participating in ternary complex formation. The 3′-CCA-Phe, the 5′ end, and the T-stem of tRNA are shown to be involved in the interaction with EF-Tu in the complex (34).

How do elongation factors work?

First, elongation factors are involved in bringing aminoacyl-transfer RNA to the ribosome during protein synthesis. Second, an elongation factor is involved in translocation, the step in elongation at which the peptidyl-tRNA is moved from one ribosomal site to another as the messenger RNA moves through the ribosome.

What happens during elongation?

During the elongation stage, the ribosome continues to translate each codon in turn. Each corresponding amino acid is added to the growing chain and linked via a bond called a peptide bond. Elongation continues until all of the codons are read. The new protein is then released, and the translation complex comes apart.

What are initiation and elongation factors?

Protein synthesis also requires a flurry of protein factors to orchestrate each step. These include initiation factors that get it all started, release factors that finish each chain, and elongation factors that assist the many steps between the beginning and the end.

What is the full form of EF TS?

EF-Ts (elongation factor thermo stable) is one of the prokaryotic elongation factors. It is found in human mitochrondria as TSFM. It is similar to eukaryotic EF-1B.

Does EF-Tu hydrolyze GTP?

In each round of ribosomal translation, the translational GTPase elongation factor Tu (EF-Tu) delivers a transfer RNA (tRNA) to the ribosome. After successful decoding, EF-Tu hydrolyzes GTP, which triggers a conformational change that ultimately results in the release of the tRNA from EF-Tu.

What is elongation rate?

Elongation is defined as the length at breaking point expressed as a percentage of its original length (i.e. length at rest). If a rubber reaches twice its length before breaking its elongation percentage is 100%.

What does elongation factor Tu Do? Elongation factor thermal unstable Tu (EF-Tu) is a G protein that catalyzes the binding of aminoacyl-tRNA to the A-site of the ribosome inside living cells. Structural and biochemical studies have described the complex interactions needed to effect canonical function. What is the function of elongation factor Ts in elongation?…